Journal: Biomedicines

Article Title: Irisin—A Pancreatic Islet Hormone

doi: 10.3390/biomedicines10020258

Figure Lengend Snippet: Confocal image of a single islet of Langerhans from ( A – C ) mouse, ( D – F ) rat and ( G – I ) human split into fibronectin type III domain-containing protein 5 (FNDC5, green), insulin (red) and a combined picture. Scale bar = 100 μm.

Article Snippet: For blocking, 3% donkey serum/PBS was used for 30 min, followed by overnight incubation at 4 °C with primary antibodies for FNDC5 (Rabbit polyclonal antibody bs-8486R, Bioss, Woburn, MA, USA) and insulin (Cat No. 20-IP30, Fitzgerald, Acton, MA, USA) or glucagon (Biotin conjugated antibody, Cat No. 13-9743-82, eBioscience™, ThermoFisher scientific, Waltham, MA, USA).

Techniques:

Journal: Biomedicines

Article Title: Irisin—A Pancreatic Islet Hormone

doi: 10.3390/biomedicines10020258

Figure Lengend Snippet: Confocal image of a single islet of Langerhans from ( A – C ) mouse, ( D – F ) rat and ( G – I ) human split into FNDC5 (green), glucagon (red) and a combined picture. Scale bar = 100 μm.

Article Snippet: For blocking, 3% donkey serum/PBS was used for 30 min, followed by overnight incubation at 4 °C with primary antibodies for FNDC5 (Rabbit polyclonal antibody bs-8486R, Bioss, Woburn, MA, USA) and insulin (Cat No. 20-IP30, Fitzgerald, Acton, MA, USA) or glucagon (Biotin conjugated antibody, Cat No. 13-9743-82, eBioscience™, ThermoFisher scientific, Waltham, MA, USA).

Techniques:

Journal: Biomedicines

Article Title: Irisin—A Pancreatic Islet Hormone

doi: 10.3390/biomedicines10020258

Figure Lengend Snippet: Relative expression of the gene fibronectin type III domain-containing protein 5 (FNDC5) in rat and human islets compared to skeletal muscle, calculated using the housekeeping genes glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and Ribosomal Protein S7 (RPS7) separately. Each value represents one animal or human donor.

Article Snippet: For blocking, 3% donkey serum/PBS was used for 30 min, followed by overnight incubation at 4 °C with primary antibodies for FNDC5 (Rabbit polyclonal antibody bs-8486R, Bioss, Woburn, MA, USA) and insulin (Cat No. 20-IP30, Fitzgerald, Acton, MA, USA) or glucagon (Biotin conjugated antibody, Cat No. 13-9743-82, eBioscience™, ThermoFisher scientific, Waltham, MA, USA).

Techniques: Expressing